Coding
Part:BBa_K863001:Design
Designed by: Isabel Huber Group: iGEM12_Bielefeld-Germany (2012-09-18)
bpul laccase from Bacillus pumilus
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 123
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 490
Illegal NgoMIV site found at 958
Illegal AgeI site found at 246
Illegal AgeI site found at 1123 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers for isolation of the gene with BioBrick Prefix in the fwd primer and Suffix in the rev primer.
fwd: 5'-ACGTGAATTCGCGGCCGCTTCTAGATGAACCTAGAAAAATTTGTT-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTATTACTGGATGATATCCATCG-3'
Source
The gene sequence was originally isolated from Bacillus pumilus DSM 27 (ATCC7061).
References
Reiss, R., J. Ihssen, et al. (2011). "Bacillus pumilus laccase: a heat stable enzyme with a wide substrate spectrum." BMC Biotechnol 11: 9.